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The older a sample is, the less (the period of time after which half of a given sample will have decayed) is about 5,730 years, the oldest dates that can be reliably measured by this process date to around 50,000 years ago, although special preparation methods occasionally permit accurate analysis of older samples.

The resulting data, in the form of a calibration curve, is now used to convert a given measurement of radiocarbon in a sample into an estimate of the sample's calendar age.Even for the first investigation, there was a possibility of using radiocarbon dating to determine the age of the linen from which the shroud was woven.The size of the sample then required, however, was ~500cm, which would clearly have resulted in an unacceptable amount of damage, and it was not until the development in the 1970s of small gas-counters and accelerator-mass-spectrometry techniques (AMS), requiring samples of only a few square centimetres, that radiocarbon dating of the shroud became a real possibility. The shroud was separated from the backing cloth along its bottom left-hand edge and a strip (~10 mm x 70 mm) was cut from just above the place where a sample was previously removed in 1973 for examination.(This, in turn, is caused by variations in the magnetic fields of the earth and sun, for example.) Although the ratio of radiocarbon to stable carbon in the atmosphere has varied over time, it is quite uniform around the globe at any given time because the atmosphere mixes very quickly and constantly.Plants obtain all their carbon atoms from the atmosphere.

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